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Polymerase chain reaction (abbreviated PCR) is a laboratory technique for rapidly producing (amplifying) millions to billions of copies of a specific segment of DNA, which can then be studied in greater detail. PCR involves using short synthetic DNA fragments called primers to select a segment of the genome to be amplified, and then multiple rounds of DNA synthesis to amplify that segment.

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Zhejiang Youlai Biotechnology Co., Ltd.

Zhejiang Youlai Biotechnology Co., Ltd. founded in May, 2020. As one of the China OEM PCR Tubes/Plates Manufacturers and OEM PCR Factory, we offer wholesale PCR for sale at factory price. My products cover three categories: 1. molecular biology research consumables; 2. laboratory consumables; 3. customization of irregular products for IVD industry.
She was born as an OEM manufacturer for world-famous brands, determine to be a leading runner in high-end lab consumables both domestically and abroad. Our founder team members have a perfect and professional understanding of the application scene. At the very beginning, we hold the craftsmanship spirit resolutely, aim to make better products and constantly satisfied customers’ thirst for higher quality.

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PCR Industry Knowledge Extension

Five Elements of PCR Reaction
There are five main substances that participate in the PCR reaction: primers (PCR primers are DNA fragments, and primers for intracellular DNA replication are RNA strands), enzymes, dNTPs, templates, and buffers (which require Mg2+).
Specific Determinants of PCR Reactions
① The specific and correct combination of primers and template DNA;
②Principles of base pairing;
③The faithfulness of Taq DNA polymerase synthesis reaction;
④ Specificity and conservation of target genes.
The correct combination of primers and templates is the key. The combination of primer and template and the extension of primer chain follow the principle of base pairing. The faithfulness of the polymerase synthesis reaction and the high temperature resistance of TaqDNA polymerase enable the combination (renaturation) of the template and the primer in the reaction to be carried out at a higher temperature, the specificity of the combination is greatly increased, and the amplified target gene fragment It can also maintain a high degree of accuracy. Then by selecting the target gene region with high specificity and conservation, the degree of specificity will be higher.
Frequently Asked Questions About PCR Reactions
1. False negative
2. Negative
3. False positives
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