What is The Difference Between a PCR Plate Without a Skirt and a Skirt?
The difference is obvious, that is, one with a skirt and one without a skirt, but the PCR plates
applicable to PCRs of different companies are different, and both have requirements for whether or not to have a skirt.
How to Arrange The PCR96-Well Plate?
96-well PCR plate
from left to right: 1 cathode K, 2 control G, 3 anode A.
If the requirements are not particularly harsh, the OD value can be measured with a spectrophotometer. Because the concentration of DNA is directly proportional to the absorbance at 260nm, as long as the OD value of the initial solution is determined, it can be added in proportion.
Ordinary PCR is mainly determined by the number of samples. If a large number of samples are made at the same time, choose 96, while quantitative PCR chooses 96, because there are usually many samples in quantification, and there is no difference between the two in good quantitative PCR. Some PCRs with gradients need to use this function to block request.
What Is a PCR Plate?
The PCR plate
is the DNA containing the desired amplified fragment. There must be a piece of DNA in it, and the primer can bind to the template DNA when it is amplified, and a new complementary strand will be synthesized under the action of the polymerase to achieve the purpose of amplification.
There are many sources of templates, such as plasmid DNA, genomic DNA, etc. extracted from samples.