PCR Tubes/Plates Manufacturers

There are five main substances that participate in the PCR reaction: primers (PCR primers are DNA fragments, and primers for intracellular DNA replication are RNA strands), enzymes, dNTPs, templates, and buffers (which require Mg2+).

① The specific and correct combination of primers and template DNA;

②Principles of base pairing;

③The faithfulness of Taq DNA polymerase synthesis reaction;

④ Specificity and conservation of target genes.

The correct combination of primers and templates is the key. The combination of primer and template and the extension of primer chain follow the principle of base pairing. The faithfulness of the polymerase synthesis reaction and the high temperature resistance of TaqDNA polymerase enable the combination (renaturation) of the template and the primer in the reaction to be carried out at a higher temperature, the specificity of the combination is greatly increased, and the amplified target gene fragment It can also maintain a high degree of accuracy. Then by selecting the target gene region with high specificity and conservation, the degree of specificity will be higher.

1. False negative

2. Negative

3. False positives