News

Home / News / 96-well PCR Tube Usage Flow

96-well PCR Tube Usage Flow

The 96-well PCR tube is a common laboratory tool used for PCR (polymerase chain reaction) amplification of DNA or RNA samples. Here is a general usage flow for the 96-well PCR tube:

Prepare the PCR reaction mixture according to the experimental design and the specific PCR kit instructions. The reaction mixture typically includes template DNA or RNA, PCR primers, nucleotides, buffer, and a DNA polymerase enzyme.

Dispense the reaction mixture into the 96-well PCR tube using a pipette or liquid handling robot. Make sure to label the tube properly to avoid confusion or misidentification during the experiment.

Add a thin layer of mineral oil or wax to the top of the reaction mixture to prevent evaporation and cross-contamination between wells.

Place the 96-well PCR tube in a thermal cycler and set the temperature and cycling conditions according to the experimental design and the specific PCR kit instructions.

Run the PCR program, which typically consists of a series of temperature cycles including denaturation, annealing, and extension. The PCR amplification will occur during these cycles.

Once the PCR program is completed, remove the 96-well PCR tube from the thermal cycler and store it at -20°C or perform further analysis such as gel electrophoresis or sequencing.

Clean and disinfect the 96-well PCR tube and all the equipment used in the experiment to prevent contamination of subsequent experiments.

Overall, the 96-well PCR tube is a valuable tool for high-throughput PCR applications and provides a convenient and efficient way to amplify DNA or RNA samples. Proper handling and usage of the tube are essential for obtaining accurate and reliable results.

We are a professional PCR Tubes Manufacturers. If you are interested, you can click the official website to contact us. We are willing to answer more information about the products for you.

Contact Us